Addenda for FISH / NGS/ and PCR Results
UT-Medical School at Houston, Pathology/ Last Revision on: 9/7/2018

>>>>List all the tests performed for each panel in Addenda

FISH PANELS

ALL FISH Profile: TCF3/PBX1 t(1;19), BCR-ABL1-ASS1 t(9;22), CDKN2A (9p21.3), KMT2A (MLL) 11q23.3, ETV6/RUNX1 t(12;21), IGH (14q32.3), TP53

ALL FISH Profile (pediatric): Cen 4,Cen 10(+4/+10), BCR-ABL1-ASS1 t(9;22), KMT2A (MLL) 11q23.3, ETV6/RUNX1 t(12;21), IGH (14q32.3)

ALL (Ph-like) for bcr-abl1-like ALL: PDGFRB (5q32), BCR/ABL1-ASS1 t(9;22), JAK2(9p24.1), EPOR (19p13.2), CRLF2(Xp22.33/Yp11.32)

AML FISH Profile: MECOM 3q26.2, DEK/NUP214 t(6;9), RUNX1T1/RUNX1 t(8;21), KMT2A (MLL) 11q23.3, PML-RARA t(15;17), CBFB/MYH11 inv(16)

CLL FISH Profile: : IGH/CCND1 t(11;14), ATM(11q22.3), Cen 12(+12), 13q(13q-/-13), TP53(17p13.1)

CML FISH Profile: BCR-ABL/ASS1 t(9;22), +8 (8cen), RARA (17q21)

Myeloma FISH Profile: CKS1B/CDKN2C (1p32.3/1q21.3), 5q(5q-/-5/+5), 13q (13q-/-13), TP53(17p13.1), IGH/FGFR3 t(4;14), IGH/CCND1 t(11;14), IGH/MAF t(14;16), IgH/MAFB t(14;20)

MDS FISH Profile (Standard): 5q(5q-/-5/+5), 7q(7q-/-7), Cen 8(+8), 20q (20q-), KMT2A(MLL) 11q23.3

Eosinophilia FISH Profile: PDBGRα (4q12), PDGFRβ (5q32), FGFR1 (8p11), JAK2 (9p24.1)

MPN FISH Profile: CKS1B/ CDKN2C (1p/1q), 5q(5q-/-5/+5), 7q(7q-/-7), Cen 8 (+8), 20q (20q-), BCR/ABL1 ASS1 t(9;22), 13q(13q-/-13)

B-NHL FISH profile (Low grade B cell): BCL6 (3q27.3), 6q(6q-/-6), MYC (8q24.21), IGH/CCND1 t(11;14), IGH/BCL2 t(14;18), MALT1 (18q21)

B-NHL High Grade FISH profile (High grade B cell): BCL6 (3q27.3), IRF4 DUSP22(6p25.3), MYC (8q24.21), IGH/BCL2 t(14;18)

MOLECULAR TESTS/PROFILES

ABL1 Kinase Domain Mutation: DNA sequence of targeted regions of ABL (exons 4, 6-7) is determined using next-generation sequencing (NGS) technology.

AML Molecular Profile 24 gene mutations by NGS (ASXL1, BCOR, CEBPA, CSF3R, DNMT3A, EZH2,IDH1, IDH2, KIT, KRAS, NPM1, NRAS, PHF6, PTPN11, RAD21, RUNX1, SF3B1, SRSF2, STAG2, TET2, TP53, U2AF1, WT1, ZRSR2)

MPN Target (Molecular) Profile (NGS): CALR, CSF3R, JAK2, MPL and SETBP1

Myeloid Molecular Profile (NGS): ASXL1, BCOR, BRAF, CALR, CEBPA, CBL, CSF3R, DNMT3A, ETV6, EZH2, GATA2, GNAS, IDH1, IDH2, JAK2, KIT, KRAS, MPL, NF1, NPM1, NRAS, PDGFRa, PDGFRb, PHF6, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5b, TET2, TP53, U2AF1, WT1 and ZRSR2

B-Cell Clonality Assessment: to detect clonal B-cell immunoglobulin heavy chain (IGH) gene rearrangement by polymerase chain reaction (PCR) of variable and joining regions on chromosome 14.

T-Cell Clonality Assessment (Gamma): T-cell receptor Gamma gene variable and joining regions on chromosome 7.