Addenda for FISH / NGS/ and PCR
Results
UT-Medical
School at Houston, Pathology/ Last Revision on: 9/6/2020
>>>>List all the tests
performed for each panel in Addenda
Rapid AML Therapeutic Panel
MOLECULAR
GENETICS:
RESULTS:
xxxx
CEBPA
Mutation: Not
Detected
FLT3
(ITD/TKD) Mutation: Not
Detected
IDH1/IDH2 Mutation: Not Detected
NPM1
Mutation: Not
Detected
TP53
Mutation: Not
Detected
FISH
ANALYSIS:
RESULTS:
xxxx
Del(5q): Not Detected
Monosomy 5: Not
Detected
Del(7q): Not Detected
Monosomy 7: Not
Detected
t(8;21): Not Detected
t(15;17): Not Detected
Del(17p) TP53: Not
Detected
KMT2A Rearrangement:
Not Detected
CBFB Rearrangement: Not
Detected
FISH PANELS
ALL,
Adult FISH Panel: TCF3/PBX1 (E2A/PBX1) t(1;19) | Trisomy or
Tetrasomy 4, 6, 10, 17 (Cen 4, Cen 6, Cen 10, Cen 17) | MYC (8q24) |
BCR/ABL1/ASS1 t(9;22) | MLL (11q23) | IgH (14q32) |
ALL, Pediatric FISH Panel: TCF3/PBX1 (E2A/PBX1) t(1;19) | Trisomy or Tetrasomy 4,
6, 10, 17 (Cen 4, Cen 6, Cen 10, Cen 17) | MYC (8q24) | BCR/ABL1/ASS1 t(9;22) |
MLL (11q23) | ETV6/RUNX1 (TEL/AML1) t(12;21) | IgH
(14q32)
ALL, Ph-like FISH Panel:
PDGFRb (5q32), BCR/ABL1-ASS1 t(9;22), JAK2 (9p24.1), EPOR
(19p13.2) and CRLF2 (Xp22.33/Yp11.32)
CLL FISH
Profile: 6q- [SEC63 (6q21), MYB (6q23)] | ATM (11q22.3) | p53 (17p13.1) | Trisomy
12 (Cen 12) | 13q-/-13 (13q14, 13q34) | CCND1/IgH
t(11;14)
Plasma
cell myeloma FISH Panel:
[1p-, 1q+, iso(1q): CDKN2C (1p32), CKS1B (1q21)
| +5, hyperdiploidy (5p15) | +9, hyperdiploidy
(9q22) | +15, hyperdiploidy (15q22) | 13q- (13q14,
13q34) | IgH (14q32) | 17p- (TP53 17p13.1, NF1
17q11.2
with reflex to IgH Complex if
positive: [FGFR3/IgH
t(4;14) | CCND1/IgH t(11;14) | IgH/MAF
t(14;16) | IgH/MAFB t(14;20)]
MDS FISH Standard Panel:
5q-, -5 (5p15, 5q31, 5q33) | 7q-, -7 (Cen 7,
7q22, 7q31) | Trisomy 8 (Cen 8) | MLL (11q23) | 20q- (20q12, 20qter)
Eosinophilia
FISH Profile: PDGFRa, CHIC2, FIP1L1 (4q12) | PDGFRb
(5q33) | FGFR1 (8p11) | CBFB inv(16), t(16;16) |
MPN FISH Panel:
PDGFRa, CHIC2, FIP1L1 (4q12) | PDGFRb
(5q33) | FGFR1 (8p11) | BCR/ABL1 t(9;22) including ASS1 (9q34)
Low grade/small B cell lymphoma
FISH panel: BCL6 (3q27) |
CCND1/IgH t(11;14) IgH/BCL2
t(14;18) | MALT1 (18q21)
High
grade/large B cell lymphoma FISH panel: BCL6 (3q27) |
MYC (8q24) | BCL2 (18q21) |
MOLECULAR TESTS/PROFILES
ABL1 Kinase Domain:
DNA sequence of targeted regions of ABL (exons 4, 6-7) is determined using
next-generation sequencing (NGS) technology.
MPN Extended Reflex Panel: JAK2 V617F, JAK2 Exon 12-14, CALR exon 9,
and MPL exon 10.
(Neotype) Myeloid Profile: ABL1, ASXL1, ATRX, BCOR, BCORL1, BRAF, CALR, CBL, CBLB, CBLC, CDKN2A,
CEBPA, CSF3R, CUX1, DNMT3A, DDX41, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1,
GATA2, GNAS, GNB1, HRAS, IDH1, IDH2, IKZF1, JAK2 including V617F and Exons
12+14, JAK3, KDM6A, KIT, KRAS, MLL, MPL, MYD88, NF1, NOTCH1, NPM1, NRAS,
PDGFRA, PHF6, PML, PPM1D, PTEN, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SH2B3,
SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, TET2, TP53, U2AF1, WT1, ZRSR2.
B-Cell Gene Rearrangement: Detection of
clonal IgH gene rearrangements by PCR of IgH framework regions 1, 2, 3 and joining regions. In
addition, Ig Kappa gene rearrangement analysis is performed using specific
oligonucleotides recognizing the Vk, intragenic and Jk regions
T-Cell Receptor Gamma:
Detection of clonal T-cell receptor gamma
(TCRG) gene rearrangements by PCR of variable and joining regions. T-Cell
Receptor Beta Gene Rearrangement is offered separately and may be added to
this gamma gene test.
T-Cell Receptor Beta:
This test provides qualitative detection of
monoclonal T-cell receptor (TCR) beta gene rearrangements by PCR and fragment
analysis according to BIOMED-2 consensus primer design. This test may be
ordered concurrently with or after negative results in T-Cell Receptor
Gamma Gene Rearrangement assay
for gamma gene rearrangements to improve TCR rearrangement detection by ~5% in
T-cell leukemias/lymphomas.