Addenda for FISH / NGS/ and PCR Results
UT-Medical School at Houston, Pathology/ Last Revision on: 9/6/2020


>>>>List all the tests performed for each panel in Addenda

 

Rapid AML Therapeutic Panel

MOLECULAR GENETICS:

RESULTS: xxxx

CEBPA Mutation: Not Detected

FLT3 (ITD/TKD) Mutation: Not Detected

IDH1/IDH2 Mutation: Not Detected

NPM1 Mutation: Not Detected

TP53 Mutation: Not Detected

 

FISH ANALYSIS:

RESULTS: xxxx

Del(5q): Not Detected

Monosomy 5: Not Detected

Del(7q): Not Detected

Monosomy 7: Not Detected

t(8;21): Not Detected

t(15;17): Not Detected

Del(17p) TP53: Not Detected

KMT2A Rearrangement: Not Detected

CBFB Rearrangement: Not Detected

 

FISH PANELS

ALL, Adult FISH Panel: TCF3/PBX1 (E2A/PBX1) t(1;19) | Trisomy or Tetrasomy 4, 6, 10, 17 (Cen 4, Cen 6, Cen 10, Cen 17) | MYC (8q24) | BCR/ABL1/ASS1 t(9;22) | MLL (11q23) | IgH (14q32) |

 

ALL, Pediatric FISH Panel: TCF3/PBX1 (E2A/PBX1) t(1;19) | Trisomy or Tetrasomy 4, 6, 10, 17 (Cen 4, Cen 6, Cen 10, Cen 17) | MYC (8q24) | BCR/ABL1/ASS1 t(9;22) | MLL (11q23) | ETV6/RUNX1 (TEL/AML1) t(12;21) | IgH (14q32)

 

ALL, Ph-like FISH Panel: PDGFRb (5q32), BCR/ABL1-ASS1 t(9;22), JAK2 (9p24.1), EPOR (19p13.2) and CRLF2 (Xp22.33/Yp11.32)

 

CLL FISH Profile: 6q- [SEC63 (6q21), MYB (6q23)] | ATM (11q22.3) | p53 (17p13.1) | Trisomy 12 (Cen 12) | 13q-/-13 (13q14, 13q34) | CCND1/IgH t(11;14)

 

Plasma cell myeloma FISH Panel: [1p-, 1q+, iso(1q): CDKN2C (1p32), CKS1B (1q21) | +5, hyperdiploidy (5p15) | +9, hyperdiploidy (9q22) | +15, hyperdiploidy (15q22) | 13q- (13q14, 13q34) | IgH (14q32) | 17p- (TP53 17p13.1, NF1 17q11.2

with reflex to IgH Complex if positive: [FGFR3/IgH t(4;14) | CCND1/IgH t(11;14) | IgH/MAF t(14;16) | IgH/MAFB t(14;20)]

 

MDS FISH Standard Panel: 5q-, -5 (5p15, 5q31, 5q33) | 7q-, -7 (Cen 7, 7q22, 7q31) | Trisomy 8 (Cen 8) | MLL (11q23) | 20q- (20q12, 20qter)

 

Eosinophilia FISH Profile: PDGFRa, CHIC2, FIP1L1 (4q12) | PDGFRb (5q33) | FGFR1 (8p11) | CBFB inv(16), t(16;16) |

 

MPN FISH Panel: PDGFRa, CHIC2, FIP1L1 (4q12) | PDGFRb (5q33) | FGFR1 (8p11) | BCR/ABL1 t(9;22) including ASS1 (9q34)

 

Low grade/small B cell lymphoma FISH panel: BCL6 (3q27) | CCND1/IgH t(11;14) IgH/BCL2 t(14;18) | MALT1 (18q21)

 

High grade/large B cell lymphoma FISH panel:  BCL6 (3q27) | MYC (8q24) | BCL2 (18q21) |

 

 

MOLECULAR TESTS/PROFILES

ABL1 Kinase Domain: DNA sequence of targeted regions of ABL (exons 4, 6-7) is determined using next-generation sequencing (NGS) technology.

 

MPN Extended Reflex Panel: JAK2 V617F, JAK2 Exon 12-14, CALR exon 9, and MPL exon 10.

 

(Neotype) Myeloid Profile: ABL1, ASXL1, ATRX, BCOR, BCORL1, BRAF, CALR, CBL, CBLB, CBLC, CDKN2A, CEBPA, CSF3R, CUX1, DNMT3A, DDX41, ETNK1, ETV6, EZH2, FBXW7, FLT3, GATA1, GATA2, GNAS, GNB1, HRAS, IDH1, IDH2, IKZF1, JAK2 including V617F and Exons 12+14, JAK3, KDM6A, KIT, KRAS, MLL, MPL, MYD88, NF1, NOTCH1, NPM1, NRAS, PDGFRA, PHF6, PML, PPM1D, PTEN, PTPN11, RAD21, RUNX1, SETBP1, SF3B1, SH2B3, SMC1A, SMC3, SRSF2, STAG2, STAT3, STAT5B, TET2, TP53, U2AF1, WT1, ZRSR2.

 

B-Cell Gene Rearrangement: Detection of clonal IgH gene rearrangements by PCR of IgH framework regions 1, 2, 3 and joining regions. In addition, Ig Kappa gene rearrangement analysis is performed using specific oligonucleotides recognizing the Vk, intragenic and Jk regions

 

T-Cell Receptor Gamma: Detection of clonal T-cell receptor gamma (TCRG) gene rearrangements by PCR of variable and joining regions. T-Cell Receptor Beta Gene Rearrangement is offered separately and may be added to this gamma gene test.

 

T-Cell Receptor Beta: This test provides qualitative detection of monoclonal T-cell receptor (TCR) beta gene rearrangements by PCR and fragment analysis according to BIOMED-2 consensus primer design. This test may be ordered concurrently with or after negative results in T-Cell Receptor Gamma Gene Rearrangement assay for gamma gene rearrangements to improve TCR rearrangement detection by ~5% in T-cell leukemias/lymphomas.